Physics Colloquium - Spring 2005 - Multiphoton imaging of intrinsic signals in brain tissue: new avenues for visualizing the activity of the brain on a cellular level
Dept of Physics & Astronomy
University of Maine, Orono, Maine
Presents
Dr. Karl Kasischke
Developmental Resource for Biophysical Imaging Optoelectronics
Cornell University, Ithaca, NY
Multiphoton imaging of intrinsic signals in brain tissue: new avenues for visualizing the activity of the brain on a cellular level
Multiphoton imaging and spectroscopy of fluorescent proteins and intrinsic fluorophores opens new avenues for examining the brain in-vivo and ex-vivo with molecular specificity and unprecedented spatial 3D-resolution. For example, we have found that two-photon fluorescence imaging of the electron-donor NADH can resolve activity-dependent metabolism in processes of astrocytes and neurons in brain tissue-slices. This functional imaging establishes a unifying model for neurometabolic coupling in which early oxidative metabolism in neurons is eventually sustained by late activation of glycolysis in astrocytes. Our model has direct implications for the design and interpretation of fMRI and PET neuroimaging studies. In addition, novel non-linear laser-microscopies such as second-harmonic generation imaging and in-vivo imaging of deep brain tissue using gradient-index (GRIN) lenses will be discussed.
Friday, February 25, 2005
3:10 pm
140 Bennett Hall
Refreshments will follow in Rm. 114, Bennett Hall
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