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Physics and Astronomy


People - Faculty - Samuel T. Hess

Sam Hess photoSamuel T. Hess
Assistant Professor of Physics
  • B.S., Physics, Yale University, 1995
  • M.S., Physics, Cornell University, 1998
  • Ph.D., Physics, Cornell University, 2002

Office: 313 Bennett Hall

Phone: (207)581-1036

EMail: Sam.T.Hess@umit.maine.edu

Other Webpages:

Research Interests:

  • Experimental and Theoretical Biophysics,
  • Fluorescence Microscopy and Spectroscopy,
  • Function and Lateral Organization of Biomembranes,
  • Single Molecule Fluorescence Photophysics,
  • Green Fluorescent Proteins.

Recent Presentations:

Recent publications:

“Nanoscale Imaging of Intracellular Fluorescent Proteins: Breaking the Diffraction Barrier,” T.J. Gould and S. T. Hess, in Methods in Cell Biology, Biophysical Tools for Biologists, Volume 2: Methods in Vivo, H. W. Detrich editor, in press

“Ultra-High Resolution Imaging of biomolecules by Fluorescence Photoactivation Localization Microscopy,” S. Hess, T. Gould, M. Gunewardene, J. Bewersdorf, and M. Mason, in Methods in Molecular Biology, J.W. Lee editor, in press

“Imaging Molecular Positions and Anisotropies,” T. J. Gould, M.S. Gunewardene, V.V. Verkhusha, J.A. Gosse, and S.T. Hess, Nature Methods 5: 1027-30, 2008

“A Quantitative Comparison of the Photophysical Properties of Select Quantum Dots and Organic Fluorophores,” T.J. Gould, J. Bewersdorf, and S.T. Hess, Z. Phys. Chem. 222: 833-849 (2008)

“Three-Dimensional sub-100 nm Resolution Fluorescence Microscopy of Thick Samples,” Manuel F. Juette, Travis J. Gould, Mark D. Lessard, Michael J. Mlodzianoski, Bhupendra S. Nagpure, Brian T. Bennett, Samuel T. Hess, and Joerg Bewersdorf, Nature Methods 5: 527-9 (2008)

“Dynamic Clustered Distribution of Hemagglutinin Resolved at 40 nm in Living Cell Membranes Discriminates Between Raft Theories,” S. T. Hess, T. J. Gould, M. V. Gudheti, S.A. Maas, K.D. Mills, and J. Zimmerberg, PNAS 104: 17370-5 (2007)

“Imaging and shape analysis of giant unilamellar vesicles (GUVs) as model plasma membranes: Effect of trans-DOPC (dielaidoyl phosphatidylcholine) on membrane properties,” M. V. Gudheti, M. Mlodzianoski, and S. T. Hess, Biophysical Journal 93: 1-13 (2007)

Hess, S.T., Gudheti, M.V., Mlodzianoski, M., and Baumgart, T., “Shape analysis of giant vesicles with fluid phase coexistence by laser scanning microscopy to determine curvature, bending elasticity and line tension,” In Methodologies to Study Membrane Lipids, Ed. A. Dopico, Methods in Molecular Biology 400: 367-87 (2007)

“Large-scale Fluid/Fluid Phase Separation of Proteins and Lipids in Giant Plasma Membrane Vesicles,” T. Baumgart, A. Hammond, P. Sengupta, S. Hess, D. Holowka, B. Baird, and W. W. Webb, PNAS 104: 3165-3170 (2007)

“Fluorescence Intermittency Limits Brightness in CdSe/ZnS Nanoparticles Quantified by Fluorescence Correlation Spectroscopy,” J. Rochira, M. Gudheti, T. Gould, R. Laughlin, J. Nadeau, and S. T. Hess, Journal of Physical Chemistry C 111: 1695-1708 (2007)

“Ultra-High Resolution Imaging by Fluorescence Photoactivation Localization Microscopy,” S. T. Hess, T.P.K. Girirajan, and M.D. Mason, Biophysical Journal 91: 4258-4272 (2006)

Hess S.T., Kumar, M., Verma, A., Farrington, J., Kenworthy, A., and Zimmerberg, J., “Quantitative Electron Microscopy and Fluorescence Spectroscopy of the Membrane Distribution of Influenza Hemagglutinin,” Journal of Cell Biology 169: 965-976 (2005).

Zimmerberg, J., Kumar, M., Verma, A., Farrington, J., Roth, M., Kenworthy, A., and S.T. Hess, “Studying spatial distributions of influenza hemagglutinin on the plasma membrane of fibroblasts: A work in progress,” Macromolecular Symposia 219: 17-23 (2004)

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Department of Physics
120 Bennett Hall
Orono, Maine 04469-5709
Phone: (207) 581-1039 | Fax: (207) 581-3410
Chairperson: Dr. David Batuski


The University of Maine
, Orono, Maine 04469
207-581-1110
A Member of the University of Maine System